Design - Analyze - Experiment - Play

Welcome to the qPCR Design Lab

Here you will find tools, information, and advice to get started with your real-time PCR/quantitative PCR (qPCR) experiments. We are sharing a productive environment providing the resources you need to design your assays with ease, and set up your experiments. There are also fun and educational aspects to this site where we discuss the history of PCR, share videos and webinars, and introduce the qPCR Crush™ game to keep you entertained in between experiment runs.

Make sure to check back regularly as we will be updating this site with the latest information about real-time PCR. If you have any feedback or suggestions for this website, please contact

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Learn about the history of PCR and the evolution of quencher chemistry and probe-based qPCR. Also review the current applications and numerous fields in which BHQ® probes and BHQplus® probes are used.

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The toolbox provides a suite of tools to assist your qPCR reactions. Get advice for multiplex dye selection and analyze the physical properties of your oligo sequences. You can also easily calculate dilutions, resuspension volumes, and the potential number of reactions from your oligo quantity.

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Create assays and evaluate qPCR probes and primers using RealTimeDesign™ Software, which is free with registration at This qPCR assay design program provides sequences for qPCR applications including gene expression analysis, SNP genotyping, and multiplexed PCR.

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A reference resource for all topics related to qPCR. Find citations, technical application notes, product posters, and guides to help fuel your research and expand your knowledge of qPCR.

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See oligos in action while watching our probe animation videos. Stay up-to-date and learn from our webinars, and other media related to qPCR.

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Take a break and blow off some steam playing qPCR Crush™ game while your samples are cycling. Post a top score to be entered for our monthly prize drawing.