Get the qPCR references you need and stand on the shoulders of giants.

Technical Application Notes:
Product Information:

real-time PCR graph

Reporting and Methods for qPCR:

Citation: The real-time polymerase chain reaction.  Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J, Lind K, Sindelka, R, Sjöback R, Sjögreen B, Strömbom L, Ståhlberg A, Zoric N. Mol Aspects Med. 2006 27: 95-125.

Citation:  Quantification of mRNA using real-time RT-PCR.  Nolan T, Hands RE, and Bustin SA. Nat Protoc. 2006;1(3):1559-82.

Citation:  Real-time PCR for mRNA quantitation.  Wong, M.L, Medrano, J.F. Biotechniques 2005 39: 75-85.

Citation:  Pitfalls of Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction. Bustin SA, Nolan T. J Biomol Tech. 2004 15:155-66.

Citation:  The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments.  Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. Clin. Chem. 2009 55(4):611-22

Citation:  Statistical aspects of quantitative real-time PCR experiment design  Kitchen RR, Kubista M, Tichopad A. Methods. 2010, 50(4):231-6.

fluorescent dyes

Probe Formats and Quenching Mechanisms:

Citation:  Fluorescence based strategies for genetic analysis.  Ranasinghe, R.T., and Brown, T. Chem. Commun. 2005 28: 5487–5502.

Citation:  Detection of specific polymerase chain reaction product by utilizing the 5’-.3’ exonuclease activity of Thermus aquaticus DNA polymerase.  Holland, P.M., Abramson, R.D., Watson, R., and Gelfand, D.H. Proc. Natl. Acad. Sci. U. S. A. 1991 88: 7276–7280

Citation:  Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes.  Marras SAE, Kramer FR and Tyagi S. Nucleic Acids Research, 2002, 30, e122.

Citation:  Intramolecular Dimers: A New Strategy to Fluorescence Quenching in Dual-Labeled Oligonucleotide Probes.  Johansson MK, Fidder H, Dick D and Cook RM. J. Am. Chem. Soc. 2002, 124, 6950.

Product Information  Probe Format Animations

 multiplex real-time PCR

BHQ Probes and Multiplexed qPCR:

Citation:  Development of an Internal Positive Control for Rapid Diagnosis of Avian Influenza Virus Infections by Real-Time Reverse Transcription-PCR with Lyophilized Reagents.  Das A, Spackman E, Senne D, Pedersen J, and Suarez DL. J Clin Microbiol. 2006; 44(9): 3065–3073.

Citation:  Measurement of gene expression in archival paraffin-embedded tissues: development and performance of a 92-gene reverse transcriptase-polymerase chain reaction assay.  Cronin M, Pho M, Dutta D, Stephans JC, Shak S, Kiefer MC, Esteban JM, Baker JB. Am J Pathol. 2004 164:35-42.

Citation:  Four-color multiplex reverse transcription polymerase chain reaction--overcoming its limitations.  Persson K, Hamby K, Ugozzoli LA. Anal. Biochem. 2005 344:33-42.

Citation:  Multiplexed detection of anthrax-related toxin genes.  Moser MJ, Christensen DR, Norwood D, and Prudent J. J Mol Diagn. 2006 Feb;8(1):89-96.

Product Info:  Dye Selection Chart  Useful tool for quickly finding the best multiplex dye options.

Product Info:  qPCR Dye Calibration Guide  Provides information about the calibration of qPCR devices to pure dye spectra.

BHQplus probes for SNP genotyping

BHQplus Probes and SNP Genotyping:

Citation:  A Multiplex Two-Color Real-Time PCR Method for Quality-Controlled Molecular Diagnostic Testing of FFPE Samples.  Yeo J, Crawford EL, Blomquist TM, Stanoszek LM, Dannemiller RE, Zyrek J, De Las Casas LE, Khuder SA, Willey JC. PLoS One. 2014 9: e89395

Citation:  Myocardial Injury and Bacterial Pneumonia Contribute to the Pathogenesis of Fatal Influenza B Virus Infection.  Paddock CD, Liu L, Denison AM, Bartlett JH, Holman RC, Deleon-Carnes M, Emery SL, Drew CP, Shieh WJ, Uyeki TM, Zaki SR. J Infect Dis. 2012 205: 895-905.

Citation:  Rapid identification of Enterobacter hormaechei and Enterobacter cloacae genetic cluster III.  Ohad S, Block C, Kravitz V, Farber A, Pilo S, Breuer R, Rorman E. J Appl Microbiol. 2014 116:1315-1321.

Citation:  Global identification of yeast chromosome interactions using Genome conformation capture.  Rodley CD, Bertels F, Jones B, and O'Sullivan JM. Fungal Genet Biol. 2009, 46(11):879-86.

Citation:  Screening for H7N9 influenza A by matrix gene-based real-time reverse-transcription PCR.  Hackett H, Bialasiewicz S, Jacob K, Bletchly C, Harrower B, Nimmo GR, Nissen MD, Sloots TP, and Whiley DM. J Virol Methods. 2014, 195:123-125.

oligonucleotide synthesis

Oligo Synthesis:

Citation:  Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method   Caruthers MH, Barone AD, Beaucage SL, Dodds DR, Fisher EF, McBride LJ, Matteucci M, Stabinsky Z, and Tang JY. Methods Enzymol. 1987, 154, 287-313

Citation:  Evaluation of New Linkers and Synthetic Methods for Internal Modified Oligonucleotides.  Walton TA, Lyttle MH, Dick DJ, and Cook, R.M. Bioconjugate Chem. 2002, 13:1155-1158.

Citation:  New Reagents and Methods for the Synthesis of Internal and 3'-Labeled DNA  Lyttle MH, Walton TA, Dick DJ, Carter TG, Beckman JH, and Cook RM. Bioconjugate Chem. 2002, 13:1146-1154.